Journal: PLoS ONE

Article Title: Substitution of Glutamate Residue by Lysine in the Dimerization Domain Affects DNA Binding Ability of HapR by Inducing Structural Deformity in the DNA Binding Domain

doi: 10.1371/journal.pone.0076033

Figure Lengend Snippet: (A) A 12-h grown culture of indicated strains were grown with agitation. Protein samples were prepared from equal number of bacterial cells, analyzed on 13.5% SDS polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently transferred to Immobilon-P membrane. FLAG-tagged HapR proteins were detected using monoclonal HRP-conjugated anti-FLAG antibody. Molecular masses were calculated with reference to SDS-PAGE molecular mass standards. (B) Coomassie-brilliant-blue-stained gel is presented as loading control for western blot experiment. Protein marker sizes are shown to the left (in kDa).

Article Snippet: Molecular masses were calculated with reference to SDS-PAGE molecular mass standards (Broad range) from NAX-GEN ALPHA PS ladder from Genetix biotech.

Techniques: Polyacrylamide Gel Electrophoresis, SDS Page, Staining, Western Blot, Marker

Journal:

Article Title: Characterization of a Red Beet Protein Homologous to the Essential 36-Kilodalton Subunit of the Yeast V-Type ATPase 1

doi:

Figure Lengend Snippet: Immuno-cross-reactivity between yeast Vma6p and beet 44-kD polypeptide. Proteins were separated on a 12% gel. Top, Immunoblot with polyclonal antiserum prepared against yeast Vma6p (αVma6p). Lane 1, Forty micrograms of whole cell protein from wild-type yeast VMA6; lane 2, 40 μg of protein from yeast vma6 mutant; and lane 3, 20 μg of red beet tonoplast protein. Relative positions of molecular mass standards are indicated. Bottom, Corresponding immunoblot with related preimmune serum.

Article Snippet: Alkaline phosphatase-conjugated antibodies and Kaleidoscope protein molecular mass standards were purchased from Promega.

Techniques: Western Blot, Mutagenesis